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GenoExplorer™ microRNA Labeling Kit
GenoExplorer™ microRNA Labeling Kit
GenoExplorer™ microRNA labeling kit is designed to label miRNAs and other small RNA molecules for microarray analysis using a simple and highly robust labeling strategy. It directly labels RNA molecules, resulting in a highly effective and selective labeling method (no enrichment required!) for both animals and plants. Combining with GenoExplorer? microRNA arrays, one can achieve
GenoExplorer™ microRNA labeling kit (patented) principle and workflow. Labeled RNA hybridizes with probes on a glass microarray slide. |
- Subfemtamole sensitivity with only 1 µg total RNA input (for optimal sensitivity we recommend using 1-2.5 µg total RNA although 100-200 ng total RNA can generate biologically significant results)
- Specificity of up to 94%
- Detection of less than 2 fold differential miRNA expression
- Uniform labeling and highly reproducible results with CV < 15% between experiments
- and > 3 logs of dynamic range on most scanners (eg. Axon, Agilent)
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Cat # Product Name Description Size Price 1301 GenoExplorer™microRNA labeling kit GenoExplorer™ microRNA labeling kit 20 reactions US$500 |
GenoExplorer™ microRNA array full kit provides a complete and easy-to-use kit with selective labeling procedure using total RNA (no enrichment required!) combined with a highly sensitive and reproducible assay. The arrays contain not only mature but precursor probes as well.
GenoExplorer™ microRNA array labeling kit directly labels RNA molecules resulting in a highly effective and selective labeling method (no enrichment required!) for both animals and plants
User Manual provides a step-by-step assay protocol. 
Technical Resources
Assay performance and technical bulletins provides expereriment data on product performance.
complete and easy-to-use kit with selective labeling procedure using total RNA (no enrichment required!) combined with a highly sensitive and reproducible assay. The arrays contain not only mature but precursor probes as well.
SELECTED RECENT PUBLICATIONS (GENOSENSOR REFERENCE)
- C Li et al. MicroRNA-29b-2-5p inhibits cell proliferation by directly targeting Cbl-b in pancreatic ductal adenocarcinoma. BMC Cancer. 2018; 18:681-94
- Ji-Su Mo et al. MicroRNA 429 Regulates Mucin Gene Expression and Secretion in Murine Model of Colitis. J Crohn’s Colitis 2016; Jan; 837-49
- Kim JM et al. Inhibition of Let7c microRNA is neuroprotective in a rat intracerebral hemorrhage model. PLoS One. 2014; Jun 24;9(6):e97946.
- Colin C. Pritchard et al. MicroRNA profiling: approaches and considerations. Nature 2012; 13:358-69
- Park JH et al. Murine hepatic miRNAs expression and regulation of gene expression in diet-induced obese mice. Mol. Cells 2011; 31:33-38.
- Foss KM et al. miR-1254 and miR-574-5p: serum-based microRNA biomarkers for early-stage non-small cell lung cancer. J Thoracic Oncol 2011; 6(3):482-8.
- Arora S et al. MicroRNA-328 is associated with (non-small) cell lung cancer (NSCLC) brain metastasis and mediates NSCLC migration. Intl J Cancer 2011; 128(11): Mar 29.
- Leeper NJ et al. MicroRNA-26a is a novel regulator of vascular smooth muscle cell function. J Cellular Physiol 2011; 226(4):1035-43.
- Lu C and Souret F. High-throughput approaches for miRNA expression analysis. Plant MicroRNAs 2010; 592:107-25.
- Mouillet JF et al. MiR-205 silences MED1 in hypoxic primary human trophoblasts. FASEB J 2010; 24(6):2030-9.
- Ranade AR et al. MicroRNA 92a-2*: a biomarker predictive for chemoresistance and prognostic for survival in patients with small cell lung cancer. J Thoracic Oncol 2010; 5(8):1273-8.
- Li G et al. Modulation of inflammatory markers by miR-146a during replicative senescence in trabecular meshwork cells. Invest Ophthalmol Vis Sci 2010; Jan 6
- Hanoun N et al. The silencing of microRNA 148a production by DNA hypermethylation is an early event in pancreatic cancinogenesis. Clin Chem 2010; Apr 29
- Liang Z et al. Involvement of miR-326 in chemotherapy resistance of breast cancer. Biochem Pharmacol 2010; 79(6):817-24
- Igoucheva O and Alexeev V MicroRNA-dependent regulation of cKit in cutaneous melanoma. Biochem Biophys Res Comm 2009; 379(3):790-4.
- Liu X et al. MicroRNA-222 regulates cell invasion by targeting matrix metalloproteinase 1 (MMP1) and manganese superoxide dismutase 2 (SOD2) in tongue squamous cell carcinoma cell lines. Cancer Genomics Proteomics 2009; 6(3):131-9.
- Nasser S et al. Identifying miRNA and imaging features associated with metastasis of lung cancer to the brain. IEEE 2009
- Blow N. Small RNAs: biology’s brave new world. Nature Methods 2009; 6(3):232-5.
- Liu X et al. microRNA-138 suppresses invasion and promotes apoptosis in head and neck squamous cell carcin. Cancer Lett2009; 286(2):217-22
- Lee S-O et al. MicroRNA15a modulates expression of the cell-cycle regulator Cdc25A and affects hepatic cystogenesis in a rat model of polycystic kidney diseases. J Clin Invest 2008; 18(11):3714-24
- Chang SS et al. miRNA alterations inhead and neck squamous cell carcinoma. Int J Cancer 2008; 123,2791-7
- Park CS and Tang SJ. Regulation of microRNA Expression by Induction of Bidirectional Synaptic Plasticity. J Mol Neurosci. 2008; Nov 8
- Goff LA et al. The analysis of microRNAs in stem cells. Stem Cell Res Therap 2008; 1:141-67.
- Chang HY et al. Microarray analysis of stem cells and their differentiation. Essential stem cell methods 2008; Chapter 12.
- Chen X-M et al. A cellular miRNA, let-7i, regulates toll-like receptor 4 expression and contributes to cholangiocyte immune responses against Cryptosporidium parvum infection. J. Biol. Chem. 2007; July 27
- Baroukh N etc. MicroRNA-124a regulates Foxa2 expression and intracellular signaling in pancreatic beta -cells lines. J Biol Chem 2007; Apr 26
- Donker RB et al. The expression of argonaute2 and related microRNA biogenesis proteins in normal and hypoxic trophoblasts. Mol Hum Reprod 2007; 13(4):273-9.
- Mott JL et al. Mir-29 regulates Mcl-1 protein expression and apoptosismer-29 downregulates Mcl-1. Oncogene 2007; 26:6133-40.
- Weston MD et al. MicroRNA gene expression in the mouse inner ear.
Brain Res 2006; 1111(1)95-104
Contact us if you want to order or have more questions.
Cat # Product Name Description Size Price
1301 GenoExplorer™microRNA labeling kit GenoExplorer™ microRNA labeling kit 20 reactions US$500
1302 GenoExplorer™microRNA labeling kit is ONLY available with purchase of full kits 4 reactions N/A