GenoExplorer™ microRNA Full Kit
GenoExplorer™ microRNA Full Kit
GenoExplorer™ microRNA full kit provides a complete and comprehensive miRNA profiling analysis tool. The kit contains a set of GenoExplorer™ microRNA chips, labeling kit, hybridization and washing buffers, and protocols for use, scanning, and data analysis. The kit is flexible and compatible with most hybridization chambers and microarray scanners. The GenoExplorer™ miRNA full kit is simple and easy to use (no enrichment required!), and chips provide updated and comprehensive miRNA information. The whole kit allows you to receive.
- The updated miRNA sequences with mature and most precursor probes all on one array. Many species are available.
- Positive and negative control probes (eg. 5S rRNA, tRNAs, U6)
- High signal to noise ratio and subfemtamole sensitivity with only 1 µg total RNA input (for optimal sensitivity we recommend using no less than 1µg total RNA although 100-200 ng total RNA can generate biologically significant results)
- Specificity of up to 94%
- Detection of less than 2 fold differential miRNA expression
- Highly reproducible results with CV < 15% between experiments
- > 3 logs of dynamic range on most scanners (eg. Axon, Agilent)
- The most convenient and cost-effective tool to meet your needs
GenoExplorer™ microRNA array products enable you to perform miRNA assays and analyses in your own laboratories. The microarray platform is flexible and compatible with most hybridization chambers and microarray scanners. The products are highly sensitive and reproducible, and provide the latest updated miRNA information.
To meet the growing demands of receiving high quality experimental data in a short time, GenoSensor provides full service microRNA expression analyses. Our experienced technical staff combined with robust experimental pipelines ensures high quality results with a fast turnaround. You can save time and money by sending your projects in for experienced handling in our laboratories.
GenoSensor’s sample-in and data-out services include database selection, probe design and synthesis, GenoExplorer™ microRNA chip fabrications, RNA labeling and processing, on-chip hybridization, post-hyb processes, chip scanning, chip gridding, data crunching, preliminary data analysis and data reports. Customers only provide the RNA samples and will receive the final experiment data.
MicroRNAs (miRNAs) are a group of small non-coding RNA molecules (19 – 23 nt) that are widely expressed in a variety of organisms in Eukaryotes and believed to negatively regulate their target transcripts. Primary miRNAs (pri-miRNAs) are much longer, they can be as long as 1000 nt, and are initially transcribed in the nucleus. They are processed into 60 – 110 nt pre-miRNAs by the dsRNA-specific RNase Drosha. The hairpin-structured pre-miRNAs are then transported to the cytoplasm and further cleaved by Dicer – a RNase III like enzyme, to a mature form of miRNAs. Mature miRNAs are 19 – 23 nt and functionally active. The single stranded miRNAs bind to their target transcripts associated with the RNA-Induced Silencing Complex (RISC) to participate in RNA interference (RNAi) regulation.
Model for miRNA Biogenesis
miRNA genes are transcribed to the primary transcripts, referred to as pri-miRNAs, which are polycistronic or monocistronic. The pri-miRNAs are processed into 60 – 110 nt pre-miRNAs by the dsRNA-specific RNase Drosha. The hairpin-structured pre-miRNAs are then exported to the cytoplasm and further cleaved by Dicer to an ~21 nt mature form of miRNAs. Mature miRNAs bind their mRNA targets associated with RNA-Induced Silencing Complex (RISC), and silence the gene expression.
There are two models for microRNA binding and gene regulations. In plants, miRNAs bind to mRNA targets by perfect or nearly perfect complementarity, and cleave the target molecules. In animals, in contrast, most miRNA-mRNA pairings are not completely complementary, resulting in translation repression or arrest without degrading the mRNA targets.
Many studies have demonstrated the involvement of miRNAs in gene regulation, cell differentiation and tissue development. MiRNAs present differential spatial and temporal expression patterns. The proposed functions of miRNAs are diverse. They may be involved in regulation of mRNA stability and translation, heterochromatin formation, genome rearrangement, and DNA excision. miRNAs may represent a new aspect of gene regulation, and much more attention has been attracted to their expression profiles, targets, and mechanisms of action.
So far hundreds of miRNA sequences have been identified in C. elegans, Drosophila, mouse, and humans. Researchers have started to investigate the roles of miRNAs in signaling pathways and gene expression regulation in the areas of developmental biology, neurological sciences, cancer, apoptosis, and immune response.