EduPrimer™ Alu DNA Profiling Kit
Catalog# 3000 – 3010
Price: $165.25
Call for additional pricing options.
The EduPrimer™ DNA Profiling Kit is specifically designed to introduce students to the principles and applications of PCR, focusing on its real-life use of DNA profiling in forensics and disease research. The core kit is designed with 30 reactions total, meant for 24 student reactions and 6 control reactions.
- Option between two dimorphic genes in the Alu family (TPA-25 and PV92) for a maximum of four shifted DNA bands per student, demonstrating the power of PCR, and teaching the techniques to utilize it.
- Extremely simple and fast protocol for easy integration into classrooms such as Introductory or General Biology. Anyone can do it.
- Quick protocol involving isolation of students’ DNA, PCR and electrophoresis, all completed within a 2.5 – 3 hour time frame.
KIT COMPONENTS INCLUDED | QUANTITY | |
DNA ISOLATION COMPONENTS | ||
Solution A (Lysis buffer) | 6 mL | included |
Solution B (Neutralizer) | 0.6 mL | included |
Cotton Swabs | 28 swabs | included |
PCR REACTION COMPONENTS | ||
2x PCR Master Mix (includes TPA25 or PV92 primers) | 300 µL | included |
Positive Control DNA (Heterozygous) | 30 µL | included |
Negative Control | 30 µL | included |
GEL ELECTROPHORESIS COMPONENTS | ||
DNA Ladder | 30 µL | included |
ADDITIONAL LAB MATERIALS NEEDED | ||
Microcentrifuge | – | |
PCR Thermal Cycler | – | |
Micropipettes & Tips | – | |
PCR Tubes | 1/sample | – |
Microcentrifuge Tubes | 1/sample | – |
Vortex | – | |
Heat Block or Water Bath | – | |
Agarose for ~0.8% – 1% gel | – | |
TAE Buffer | – | |
DNA Dye (recommended in gel, UV visualized dye) | – | |
Electrophoresis Apparatus | – | |
UV Lamp / UV Light Box | – | |
Scissors and Tweezers | – | |
| Cat # | Product Name | Description | Size | List Price |
|---|---|---|---|---|
3000 | EduPrimer™ DNA Isolation Kit | DNA Isolation components only (Solution A, B and Swabs) | 30 rxns | $55 |
3001 | EduPrimer™ DNA Profiling Kit – TPA-25 | Full kit, as detailed in kit components, using TPA-25 primers | 30 rxns | $165.25 |
3001R | EduPrimer™ PCR Reaction Kit – TPA-25 | PCR reaction components only using TPA-25 primers. | 30 rxns | $125 |
3001C | Positive Control for EduPrimer™ DNA Profiling Kit – TPA-25 | Extra positive control DNA for TPA-25 | 10 rxns | $30 |
3002 | EduPrimer™ DNA Profiling Kit – PV92 | Full kit, as detailed in kit components, using PV92 primers | 30 rxns | $165.25 |
3002R | EduPrimer™ PCR Reaction Kit – PV92 | PCR reaction components only using PV92 primers. | 30 rxns | $125 |
3002C | Positive Control for EduPrimer™ DNA Profiling Kit – PV92 | Positive control DNA for PV92 | 10 rxns | $30 |
3010 | DNA ladder | Extra DNA ladder | 100 µL | $42 |
User Manual 
Single Page Protocol
VIDEOS
We’ve put together short, 4 1/2 minute video showing kit components and the protocol:
Arizona State University also made a video describing their experience with the kit:
REFERENCES
Detection of Alu by PCR: A Human DNA Fingerprinting Lab Protocol. DNA Learning Center. Cold Spring Harbor Laboratory. 1994.
Pray, Leslie A. Functions and Utility of Alu Jumping Genes. http://www.nature.com/scitable/topicpage/functions-and-utility-of-alu-jumping-genes-561. Nature Education (2008)
The EduPrimer™ DNA Profiling Kit is specifically designed to introduce students to the principles and applications of PCR, focusing on its real-life use of DNA profiling in forensics and disease research. The core kit is designed with 30 reactions total, meant for 24 student reactions and 6 control reactions.
- Option between two dimorphic genes in the Alu family (TPA-25 and PV92) for a maximum of four shifted DNA bands per student, demonstrating the power of PCR, and teaching the techniques to utilize it.
- Extremely simple and fast protocol for easy integration into classrooms such as Introductory or General Biology. Anyone can do it.
- Quick protocol involving isolation of students’ DNA, PCR and electrophoresis, all completed within a 2.5 – 3 hour time frame.
KIT COMPONENTS INCLUDED | QUANTITY | |
DNA ISOLATION COMPONENTS | ||
Solution A (Lysis buffer) | 6 mL | included |
Solution B (Neutralizer) | 0.6 mL | included |
Cotton Swabs | 28 swabs | included |
PCR REACTION COMPONENTS | ||
2x PCR Master Mix (includes TPA25 or PV92 primers) | 300 µL | included |
Positive Control DNA (Heterozygous) | 30 µL | included |
Negative Control | 30 µL | included |
GEL ELECTROPHORESIS COMPONENTS | ||
DNA Ladder | 30 µL | included |
ADDITIONAL LAB MATERIALS NEEDED | ||
Microcentrifuge | – | |
PCR Thermal Cycler | – | |
Micropipettes & Tips | – | |
PCR Tubes | 1/sample | – |
Microcentrifuge Tubes | 1/sample | – |
Vortex | – | |
Heat Block or Water Bath | – | |
Agarose for ~0.8% – 1% gel | – | |
TAE Buffer | – | |
DNA Dye (recommended in gel, UV visualized dye) | – | |
Electrophoresis Apparatus | – | |
UV Lamp / UV Light Box | – | |
Scissors and Tweezers | – | |
| Cat # | Product Name | Description | Size | List Price |
|---|---|---|---|---|
3000 | EduPrimer™ DNA Isolation Kit | DNA Isolation components only (Solution A, B and Swabs) | 30 rxns | $55 |
3001 | EduPrimer™ DNA Profiling Kit – TPA-25 | Full kit, as detailed in kit components, using TPA-25 primers | 30 rxns | $165.25 |
3001R | EduPrimer™ PCR Reaction Kit – TPA-25 | PCR reaction components only using TPA-25 primers. | 30 rxns | $125 |
3001C | Positive Control for EduPrimer™ DNA Profiling Kit – TPA-25 | Extra positive control DNA for TPA-25 | 10 rxns | $30 |
3002 | EduPrimer™ DNA Profiling Kit – PV92 | Full kit, as detailed in kit components, using PV92 primers | 30 rxns | $165.25 |
3002R | EduPrimer™ PCR Reaction Kit – PV92 | PCR reaction components only using PV92 primers. | 30 rxns | $125 |
3002C | Positive Control for EduPrimer™ DNA Profiling Kit – PV92 | Positive control DNA for PV92 | 10 rxns | $30 |
3010 | DNA ladder | Extra DNA ladder | 100 µL | $42 |
User Manual
Single Page Protocol
VIDEOS
We’ve put together short, 4 1/2 minute video showing kit components and the protocol:
Arizona State University also made a video describing their experience with the kit:
REFERENCES
Detection of Alu by PCR: A Human DNA Fingerprinting Lab Protocol. DNA Learning Center. Cold Spring Harbor Laboratory. 1994.
Pray, Leslie A. Functions and Utility of Alu Jumping Genes. http://www.nature.com/scitable/topicpage/functions-and-utility-of-alu-jumping-genes-561. Nature Education (2008)